MAGicBead cfDNA Isolation Kit, 2mLx50 preps

The MAGicBead cfDNA Isolation Kit features a unique, magnetic bead surface chemistry that facilitates unparalleled cfDNA recovery from biofluids. Input volumes can range up to 10 mL of plasma. It is compatible with all open automation platforms and a wide range of sample types serum, saliva, urine, etc.. This kit features a simple, streamlined extraction process with minimal number of steps and does not require the bead drying step common with other similar procedures. Eluted cfDNA is free of enzymatic inhibitors that can interfere with PCR or other sensitive downstream applications, including Next-Gen Sequencing.Highest Yield: Robust cfDNA isolation from up to 10 mL plasma w/ unique MAGicBead surface technologyNGS-Ready: High-quality cfDNA from plasma, saliva, urine, etc. is ideal for NGSCompatible: With all open automation platformsElution Volume: 15 l MAGicBead cfDNA Elution Buffer.Fragment Sizes: For plasma samples, DNA fragments will typically display a mono-nucleosomal peak in the 140-170 bp range and a genomic DNA fragments peak > 10 kb. However, other multi-nucleosomal apoptotic DNA fragments may also be present.Processing Time: 1-1.5 hour 15 minutes hands-on time.Purity: Eluted cfDNA is NGS-ready. NOTE: Due to low levels of cfDNA available in cell-free biofluids, a spectrophotometer e.g., NanoDrop is not recommended for quantification or purity assessment.Required Equipment: An open automation platform: KingFisher Flex/Apex Thermo-Fisher Scientific, Fluent X Tecan, Microlab STAR Hamilton, OT-2 Opentrons and others. For manual applications, a magnetic stand compatible with conical/centrifuge tubes/plates and a rotator capable of at least 30 rpm are needed.Sample Compatibility: Plasma samples derived from most blood collection tube types are compatible, including Streck DNA BCT. Plasma samples derived from blood collection tubes with heparin are NOT compatible. Highly viscous biofluids, such as whole saliva and synovial fluids, may have limited compatibility. Refer to Appendix A Page 6 for more details.Yield: Typical yields range from 0.5 ng to 20 ng from healthy donor plasma samples. This can range from 0.1 ng to 100 ng depending on the health status of donors.Shipping Weight: 0.0626 lbsResearch or further manufacturing use only, not for food or drug use.