Quick-16S Plus NGS Library Prep Kit V1-V2

The Quick-16S Plus NGS Library Prep Kit V1-V2 is the fastest and simplest NGS library prep targeting the V1-V2 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a single qPCR/PCR for combined targeted amplification and barcode addition using specially designed primers. After pooling by equal volume, a single clean-up of the final library is performed, rather than massive AMPure bead-based clean-ups. Additional library quantification analysis such as TapeStation analysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes. 16s V1-V2 Primer Sequences adapters not included: 27f AGRGTTYGATYMTGGCTCAG, 20 bp and 341r CTGCWGCCHCCCGTAGG, 17 bp.Amplicon Size: The final amplicon size after 1-Step PCR targeted amplification and barcode addition is 492 bp.Barcode Sequences: 10 bp barcodes are available to download here or by visiting the Documentation section of this Product Page.Index Primers: Unique dual index barcodes to label individual samples.Required Equipment: Microcentrifuge, plate spinner centrifuge, 96-well real-time quantitative PCR system SYBR Green compatible or standard PCR system, and 96-well real-time PCR plates.Sample Input: Purified microbial DNA 100 ng, free of PCR inhibitors.Sequencing Platform: Illumina MiSeq or NextSeq 1000/2000 without the need to add custom sequencing primers. We recommend the MiSeq Reagent Kit v3 600-cycle or NextSeq 1000/2000 Reagent Kit P1/P2 600-cycle. For assistance with sample sheet setup, see Appendix F.Shipping Weight: 2.2 lbsShipping Dimensions: 4.00 x 2.75 x 6.00Research or further manufacturing use only, not for food or drug use.