Quick-16S Plus NGS Library Prep Kit V3-V4, UDI, w/Primer Set 2, Rxns 96

The Quick-16S Plus NGS Library Prep Kit V3-V4, UDI is the fastest and simplest NGS library prep targeting the V3-V4 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a single qPCR/PCR for combined targeted amplification and barcode addition using specially designed primers. After pooling by equal volume, a single clean-up of the final library is performed, rather than massive AMPure bead-based clean-ups. Additional library quantification analysis such as TapeStation analysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.The most streamlined NGS kit with only 30 minutes of hands-on time for 96 samples.100% automation ready with only a single PCR step and without the need for normalization.Real-time PCR enables absolute microbial copy number quantification.16S V3-V4 Primer Sequences adapters not included: 341f CCTACGGGDGGCWGCAG, CCTAYGGGGYGCWGCAG, 17 bp and 806r GACTACNVGGGTMTCTAATCC, 24 bp. The forward primer 341f is a mixture of the two sequences listed.Amplicon Size: The final amplicon size after 1-Step PCR targeted amplification and barcode addition is 606 bp.Barcode Sequences: Barcode Sequences: 10 bp barcodes, Available for download here.Index Primers: Unique dual index barcodes to label individual samples.Primer Coverage: bacteria 84.2%, archaea 75.8%, and eukaryota 0.0%.Required Equipment: Microcentrifuge, plate spinner centrifuge, 96-well real-time quantitative PCR system SYBR Green compatible or standard PCR system, and 96-well real-time PCR plates.Sample Input: Purified microbial DNA 100 ng, free of PCR inhibitors.Sequencing Platform: Illumina MiSeq without the need to add custom sequencing primers. Zymo Research recommends the MiSeq Reagent Kit v3 600-cycle. For assistance with sample sheet setup, see Appendix F.Shipping Weight: 0.0626 lbsResearch or further manufacturing use only, not for food or drug use.