Quick-16S Plus NGS Library Prep Kit V4 with Primer Set 2, 96rxns

The Quick-16S Plus NGS Library Prep Kit V4 is the fastest and simplest NGS library prep targeting the V4 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a premixed amplification plate and a single qPCR/PCR for combined targeted amplification and barcode addition. Pooling by equal volume and a single clean-up of the final library, rather than massive AMPure bead-based clean-ups, simplifies the library prep process. Additional library quantification analysis such as TapeStation analysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.Fast: 8x less hands-on time than conventional 16S library prep protocols.Easy: Premixed Plate minimizes hands-on time and handling errors.Simple Workflow: 100% Automation ready with only a single PCR step and no need for normalization. Just pool by equal volume!Amplicon Size The final amplicon size after 1-Step PCR targeted amplification and barcode addition is 388 bp.Equipment Needed user provided Microcentrifuge, plate spinner centrifuge, 96-well real-time quantitative PCR system SYBR Green compatible recommended, or standard PCR system, and 96-well real-time PCR plates.Index Sequences 10 bp indexes are listed in the MiSeq Sample Sheet Template provided.Sample Input Purified microbial DNA 100 ng, free of PCR inhibitors.Sequencing Platform Compatible with all Illumina sequencing platforms using custom sequencing primers. We recommend the MiSeq Reagent Kit v2 300-cycle.V4 Primer Sequences adapters not included 515f GTGYCAGCMGCCGCGGTAA and 806r GGACTACNVGGGTWTCTAAT.Shipping Weight: 1 lbsResearch or further manufacturing use only, not for food or drug use.