CpG Methylase, M. Sssl, 400 U

CpG Methylase completely methylates all cytosines in double-stranded, non-methylated, and hemimethylated DNA in a 5'...CpG...3' context. The recombinant methylase is isolated from an E. coli strain that expresses the methyltransferase gene from Spiroplasma sp. strain MQ1. Reaction conditions have been optimized to maximize the processivity of the enzyme to ensure rapid, complete, and reproducible methylation of DNA for accurate DNA methylation analysis. This product is supplied with 10X Reaction Buffer and S-adenosylmethionine cofactor.For complete, in vitro methylation of DNA for methylation analysis. Methylation of chromatin DNA for DNA accessibility studies. Inhibition of endonucleases with overlapping CpG sequence recognition and [3H]-labeling of DNA.
Additional Information: Enzyme Commission Number (EC 2.1.1.37) Assay Condition: CpG Methylase in 1X CpG Reaction Buffer w/ 600 μM SAM. Incubate reaction mixtures at 30C (Performing the Methylase reaction at 30°C rather than 37°C promotes optimal reaction kinetics). Heat Inactivation: 65°C for 20 minutes Source: Recombinant methylase is isolated from E. coli expressing the methyltransferase gene from Spiroplasma sp. strain MQ1. Storage Store: at -20°C for up to 12 months. Avoid repeated freeze/thaw cycles of reagents. Prolonged storage is at -70°C. Unit Definition: One unit (U) is the amount of enzyme required to protect 1μg of λ DNA from cleavage by BstUI restriction endonuclease in a total reaction volume of 20 μl for 1 hour at 37°C.Shipping Weight: 0.125 lbs
Shipping Dimensions: 5.30 x 3.30 x 1.00
Research or further manufacturing use only, not for food or drug use.