DNA Degradase, 500 Units

DNA Degradase from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleotide components. DNA Degradase is ideal for whole-genome DNA methylation analysis by a number of downstream applications (i.e., HPLC, TLC, etc.). Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods. Quick and simple procedure for completely degrading DNA into its individual nucleotide component for quantitative analysis (e.g., whole-genome methylation analysis by HPLC, TLC, etc.)1 hour, single-enzyme digest vs. conventional 6 - 16 hour multi-step enzyme digestion protocols
Storage Conditions: Freezer
Format: Provided in solution (10 units/μl) w/ 10X Reaction Buffer Storage: Store at -20°C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage should be -70°C. Unit Definition: One unit (1 U) is defined as the amount of enzyme required to degrade 1 μg of λ DNA in a total reaction volume of 25 μl for 1 hour at 37°C. Reaction Conditions: DNA Degradase in 1X DNA Degradase Reaction Buffer. Incubate reaction mixtures at 37°C for 1 hour. Inactivation: Heat-inactivate at 70°C for 20 minutes.Shipping Weight: 0.125 lbs
Shipping Dimensions: 1.00 x 1.00 x 2.00
Research or further manufacturing use only, not for food or drug use.