DNA Degradase Plus from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleoside components. Since nucleosides lack negatively charged phosphate, DNA Degradase Plus is ideal for whole-genome DNA methylation analysis by LC/MS. Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods. Quick and simple procedure for generating single nucleosides from DNA for quantitative analysis via LC/MS Convienent 2 hour, single enzyme digest v. conventional 6-16 hr multi-step enzyme digestion protocolAssay Condition: DNA Degradase Plus in 1X DNA Degradase Reaction Buffer. Incubate reaction mixtures at 37C for 1 hourConcentration: 5 U/μl Enzyme Inactivation: Heat inactivate enzyme at 70C for 20 minutesStorage: Store at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at -70C. Unit Definition: 5 U of enzyme will digest 1 μg of genomic DNA in 25 μl reaction volume at 37C for 1 hrShipping Weight: 0.0626 lbsShipping Dimensions: 1.00 x 2.00 x 2.00Research or further manufacturing use only, not for food or drug use.