DNase I Set

DNase I (RNase-free) cuts both double-stranded and single-stranded DNA, producing 3€2-OH oligonucleotides. It is typically used for selectively degrading DNA in the presence of RNA. This DNase is suited for applications such as nick translation, production of random fragments, cleavage of genomic DNA for footprinting, removal of DNA template after in vitro transcription, and removal of DNA from RNA samples prior to applications such as RT-PCR. It is compatible with all of our RNA kits featuring in-column DNase digestion. The features lyophilized enzyme and DNA Digestion Buffer. Lyophilized enzyme provided with DNA Digestion Buffer
Storage Temperature: -20C
Storage Conditions: Freezer
Concentration: 1 U/μl Heat inactivation: Add a final concentration of 5 mM EDTA, then incubate at 65°C for 10 min.. Included: One unit (U) is defined as the amount of enzyme required to degrade 1 μg λ DNA completely in 10 minutes at 37°C in a 50 μl reaction volume (40 mM Tris-HCl, pH 8.0, 10 mM NaCl, 6 mM MgCl2, and 10 mM CaCl2). One unit of enzyme is equivalent to one Kunitz unit. Resuspension Volume: 275 μlSize: 250 UShipping Weight: 0.5 lbs
Shipping Dimensions: 5.50 x 3.50 x 2.00
Research or further manufacturing use only, not for food or drug use.