E. coli Non-Methylated Genomic DNA (5 µg/ 20 µl)

E. coli non-methylated genomic DNA is from a Dam€“ and Dcm€“ strain (ER2925) of E. coli. It works perfectly as a negative control for DNA methylation analyses requiring DNA with absolutely no methylation.Negative control for epigenetic experiments requiring DNA containing zero background levels of cytosine and adenosine methylation. Substrate for DNA methylation-sensitive restriction enzyme digestions.
Storage Temperature: -20C
Storage Conditions: Freezer
Concentration: 250 ng/μl in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0)Genotype: ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2 Storage: -20°CShipping Weight: 0.125 lbs
Shipping Dimensions: 5.30 x 3.30 x 1.00
Research or further manufacturing use only, not for food or drug use.