EZ-96 DNA Methylation-Gold MagPrep, 4 x 96 rxns

The EZ-96 DNA Methylation-Gold MagPrep integrates DNA denaturation and bisulfite conversion processes into one step followed by a magnetic bead-based clean-up for high-throughput methylation analysis. To streamline the procedure, high temperature is used instead of sodium hydroxide to denature DNA. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. The EZ DNA Methylation-Gold kits have been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for downstream analyses, including PCR amplification, endonuclease digestion, sequencing, microarrays, etc. Complete, high-throughput bisulfite conversion of GC-rich DNA in less than 3 hours. A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines to uracil.High throughput (96-well), automated desulphonation and recovery of bisulfite-treated DNA. Eluted, ultra-pure DNA is ideal for use in subsequent molecular-based analyses.
Storage Temperature: Room Temperature
Storage Conditions: Room Temperature
Applications: Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc. Conversion: > 99%Elution Volume: 25 μlEquipment: Thermocycler with heated lid, heating element for 96-well plate, magnetic stand.Input: 500 pg - 2 μg of DNA. Processing Time: 3 hours Recovery: > 70% Sample Source: Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.Shipping Weight: 3.0001 lbs
Shipping Dimensions: 13.00 x 8.00 x 6.50
Research or further manufacturing use only, not for food or drug use.