EZ-96 DNA Methylation MagPrep, 8 x 96 Rxns

The EZ-96 DNA Methylation MagPrep is a bisulfite conversion kit that features a high-throughput (96-well), simplified procedure that streamlines bisulfite conversion of DNA. The kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite during which cytosine is converted into uracil. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.Proven procedure for complete bisulfite conversion of DNA.High-throughput (96-well), automated desulphonation and recovery of bisulfite-treated DNA. Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
Storage Temperature: Room Temperature
Storage Conditions: Room Temperature
Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc.Conversion > 99%Equipment Thermocycler with heated lid, heating element for 96-well plate, magnetic stand.Input 500 pg - 2 g of DNAProcessing Time 12-16 hoursRecovery > 70%Sample Source Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.Shipping Weight: 8.3749 lbs
Shipping Dimensions: 13.00 x 8.50 x 13.00
Research or further manufacturing use only, not for food or drug use.