EZ DNA Methlyation-Direct Kit, 200 Reactions

The EZ DNA Methylation-Direct kit is a bisulfite conversion kit that features reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of this bisulfite conversion kit makes it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. Recovered bisulfite-converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc. Complete bisulfite conversion of DNA directly from blood, soft tissue, cells, FFPE samples, and LCM samples.Compatible with small sample inputs as few as 10 cells or 50 pg of DNA. DNA recovered by this bisulfite conversion kit is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
Storage Temperature: Room Temperature
Storage Conditions: Room Temperature
Applications: Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc.Conversion: > 99.5% Elution Volume: 10 μl Equipment: Thermocycler with heated lid and microcentrifuge.Input: Samples containing between 50 pg to 2 μg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. The number of cells per standard treatment can range from 10-105 cells.Processing Time: 4 hours Recovery: > 80%Sample Source: Blood, tissue, cells, FFPE, LCM-derived samples, purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc.Shipping Weight: 4.0001 lbs
Shipping Dimensions: 11.00 x 8.00 x 6.00
Research or further manufacturing use only, not for food or drug use.