EZ DNA Methylation-Gold Kit, 200 Rxns

The EZ DNA Methylation-Gold Kit is a refinement of our popular EZ DNA Methylation kit and uses heat denaturation instead of chemical denaturation of the input DNA. This allows for the denaturation and bisulfite conversion steps to be consolidated into one step, leading to a much-reduced incubation time. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc. Complete bisulfite conversion of DNA in less than 3 hours. Desulphonation and recovery of bisulfite-treated DNA with a spin column. Recovered DNA is ideal for downstream analyses such as PCR, endonuclease digestion, sequencing, microarrays, etc.
Storage Temperature: Room Temperature
Storage Conditions: Room Temperature
Applications: Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. Conversion: >99%Elution Volume: 10 μl Equipment: Microcentrifuge and thermocycler with heated lid Input: 500 pg - 2 μg of DNA. Processing Time: 3 hours Recovery: >75% Sample Source: Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.Shipping Weight: 4.0001 lbs
Shipping Dimensions: 11.00 x 8.00 x 6.00
Research or further manufacturing use only, not for food or drug use.