EZ DNA Methylation Kit, 200 Rxns

The EZ DNA Methylation Kit features a simplified procedure that streamlines bisulfite treatment of DNA. The EZ DNA Methylation Kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite during which cytosine is converted into uracil. Innovative desulphonation technologies eliminate otherwise cumbersome precipitations. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including library preparation for Next-generation sequencing, PCR amplification, endonuclease digestion, sequencing, microarrays, etc. The EZ DNA Methylation Kit is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.Streamlined, proven procedure for bisulfite conversion of DNA. Desulphonation and recovery of bisulfite-treated DNA with a spin column. Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
Storage Temperature: Room Temperature
Storage Conditions: Room Temperature
Applications: Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. This kit is recommended for use with Illumina Infinium MethylationEPIC BeadChip array. Conversion: >99%Elution Volume: 10 μl Equipment: Microcentrifuge and thermocycler with heated lid Input: 500 pg - 2 μg of DNA Processing Time: 12-16 hoursRecovery:>80% Sample Source: Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.Shipping Weight: 3.0001 lbs
Shipping Dimensions: 12.00 x 9.00 x 6.00
Research or further manufacturing use only, not for food or drug use.