HostZERO Microbial DNA Kit

The is designed to overcome the challenge of contaminating host nucleic acids in microbial samples. This kit uses a novel method to reduce the amount of contaminating host DNA by selectively lysing the eukaryotic cells and degrading this DNA prior to total DNA purification. Paired with Zymo Research€s non-biased purification technology, the allows for the exclusive capture of DNA from living microbial cells in a biological sample. This new technology is able to reduce the presence of human DNA in a saliva sample from 65% (untreated sample) to less than 1% (treated sample). Concurrently, the depletion process utilizes the ZymoBIOMICS non-biased DNA isolation technology for accurate data representation. Finally, the recovers the highest amount of bacterial DNA as compared to other methods. By removing the presence of host DNA and reducing bias in purification, the produces the highest-quality data for microbial samples.Depletes Host and Enriches for Bacteria: Increase the ratio of bacteria to host DNA. Fast: Only 30 minutes of hands-on time for purification of high-quality DNA. Accurate: Unbiased cellular lysis for accurate analysis of the microbial profile.
Storage Temperature: Room Temperature
Storage Conditions: Room Temperature
DNA Purity: High-quality DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation Sequencing. DNA Recovery: Greater than 85% of total bacterial DNA is effectively recovered with greater than 90% of eukaryotic host DNA depleted. Processing Time: Bacterial DNA is isolated from a single sample in less than 90 minutes. Required Equipment: Microcentrifuge, vortex, tube rotator, and high-speed cell disruptor (recommended).Sample Input: Up to 200 μl liquid sample. Sample Type: Saliva, swabs, and bodily fluids from eukaryotic hosts with intact (living) bacteria cells.Shipping Weight: 1 lbs
Shipping Dimensions: 4.00 x 4.00 x 6.00
Research or further manufacturing use only, not for food or drug use.