Mix & Go! Competent Cells-JM109, 96 x 50 µl

The Mix & Go! E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go! E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/ g pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.
Additional Info: Partly restriction-deficient; good strain for cloning repetitive DNA (recA-). Suppresses many amber mutations when glutamine is available but not the S100 or S7 mutation of λ, e.g.,λgt11. Can be used for M13 cloning/sequencing and blue/white screening. Genotype: F`[traD36 proA+B+ laclq Δ(lacZ)M15] Δ(lac-proAB) glnV44 (supE44) e14- (McrA-) thi gyrA96 (NalR) endA1 hsdR17(rk- mk+) relA1 recA1Processing Time: 20 SecondsProduct Storage: -70°C to -80°C Transformation Efficiency: 108 - 109 transformants per μg of plasmid DNAShipping Weight: 1.06 lbs
Shipping Dimensions: 5.00 x 3.30 x 2.00
Research or further manufacturing use only, not for food or drug use.