Quick-cfDNA/cfRNA Serum & Plasma Kit, 50/preps

The Quick-cfDNA/cfRNA Serum & Plasma Kit enables simple and efficient preparation of all circulating cell-free DNA and RNA (including protein-bound, exosomal, microRNA, and other small RNA) from serum, plasma, and other biological fluids, without using toxic phenol-chloroform or cumbersome protein precipitation and removal steps. The kit efficiently separates cell-free DNA and RNA, with option to co-elute. Cell-free nucleic acids scale linearly relative to the sample input volume up to 3 ml. Zymo-Spin Technology enables isolation of ultra-pure cell-free nucleic acids suitable for all subsequent analyses, such as RT-qPCR and NGS.Quick & Easy: Simple spin-column based isolation from up to 3 mL of plasma, serum, or other biological liquids. No phenol/chloroform or precipitation.Highest Yields: Efficient isolation of cell-free DNA and RNA. Recover up to 515 times more microRNA. Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, nCounter, etc.
Storage Temperature: Room Temperature
Storage Conditions: Room Temperature
Elution Volume: cfDNA and cfRNA can be eluted into 15 μl (to maximize total yield) or as little as 6 μl (for highly concentrated eluate) of DNase/RNase-Free Water.Processing Time: Typically requires 30 minutes (cfDNA/cfRNA co-elution protocol) to 45 minutes (cfDNA/cfRNA separation protocol) hands-on time to process 10 samples. Sample digestion requires 2 hours. Purity: High quality cell-free DNA (cfDNA) and cell-free RNA (cfRNA) are ready for all sensitive downstream applications such as RT-qPCR and Next-Generation Sequencing. Required Equipment: Microcentrifuge, vortex, water bath or heating block, vacuum, and vacuum manifold.Sample Types: Up to 3 ml of plasma, serum, cerebrospinal fluids, amniotic fluids, urine, or other biological fluids. Size: DNA 50 bp and RNA 17 nt. Yield: Yields are sample dependent and may vary. Typical recovery ranges from 1 €“ 100 ng/ml of human plasma or serum for both cfDNA and cfRNA. Kit is optimized for recovery of total cfDNA and cfRNA, including small RNA and microRNA.Shipping Weight: 4.1251 lbs
Shipping Dimensions: 7.00 x 5.00 x 5.00
Research or further manufacturing use only, not for food or drug use.