Quick-cfRNA Serum & Plasma, 50 Preps

The Quick-cfRNA Serum & Plasma Kit enables simple, reliable, and efficient preparation of high-quality circulating cell-free RNA (including protein-bound, exosomal, miRNA and other small RNAs) from serum, plasma and other biological fluids. The kit does not use toxic phenol-chloroform or cumbersome protein precipitation and removal steps. Recovered cell-free RNA scales linearly relative to the sample input volume up to 3 ml. ZymoSpin Technology enables isolation of ultra-pure cell-free RNA suitable for all subsequent analyses, such as RT-qPCR and NGS. Empower your discovery of circulating RNA biomarkers. Quick & Easy: Simple spin-column based isolation from up to 3 mL of plasma, serum, or other biological liquids. No phenol/chloroform or precipitation. Highest Yields: Efficient isolation of total cell-free RNA. Recover up to 515 times more microRNA. Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, nCounter, etc.
Storage Temperature: Room Temperature
Storage Conditions: Room Temperature
Elution Volume: cfRNA can be eluted into 15 μl (to maximize total yield) or as little as 6 μl (for highly concentrated eluate) of DNase/RNase-Free Water. Processing Time: Typically requires 30 minutes hands-on time to process 10 samples. Sample digestion requires 2 hours.Purity: High quality cell-free RNA (cfRNA) is ready for all sensitive downstream applications such as RT-qPCR and Next-Generation Sequencing. Required Equipment: Microcentrifuge, vortex, water bath or heating block, vacuum, and vacuum manifold. Sample Type: Up to 3 ml of plasma, serum, cerebrospinal fluids, amniotic fluids, urine, or other biological fluids. Size: RNA 17 nt. Yield: Yields are sample dependent and may vary. Typical recovery ranges from 1 €“ 100 ng/ml of human plasma or serum. Kit is optimized for recovery of total cfRNA, including small RNA and microRNA.Shipping Weight: 4.5001 lbs
Shipping Dimensions: 13.00 x 8.00 x 6.50
Research or further manufacturing use only, not for food or drug use.