ZR BAC DNA Miniprep Kit, 100 Preps

The ZR BAC DNA Miniprep Kit efficiently isolates BAC plasmid DNA or other large plasmids (e.g., PAC) from E. Coli using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol with color-coded reagents that allow easy visualization of complete bacterial cell lysis and neutralization. The innovative Zymo-Spin IC-XL columns are optimized for high yield, endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is ideal for sequencing, PCR, endonuclease digestion, etc. High Purity: Purify endotoxin-free BAC/PAC plasmid DNA (up to ~200 kb) for sequencing, PCR, restriction endonuclease digestion, etc. Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralizationUnique column design: Zero buffer retention and low elution volume (10 μl
Storage Temperature: Room Temperature
Storage Conditions: Room Temperature
Applicable For: Transfection, transformation, sequencing, restriction endonuclease digestion, PCR, in vitro transcription, and other sensitive applications.Elution Volume: 10 μl Equipment: Microcentrifuge Processing Time: 15 min Purity: Typical Abs 260/280 1.8 and Abs 260/230 2.0Size Range: Up to ~200 kb Yield: Up to 10 μg per preparation, depending on the plasmid copy number, initial volume of E. Coli culture processed, and culture growth conditions. The typical yield is 1 μg per 3 ml of culture.Shipping Weight: 2 lbs
Shipping Dimensions: 4.00 x 4.00 x 6.00
Research or further manufacturing use only, not for food or drug use.