Zymo Research

ZymoBIOMICS MagBead RNA, 4x96 preps

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The ZymoBIOMICS MagBead RNA kit provides a high-throughput, magnetic beadbased purification of total RNA (including small/microRNAs) from any sample source (e.g., microbes including gram positive/negative bacteria, fungi, protozoans, viruses, fecal, soil, plant, water, biofilms, swabs, saliva, body fluids and etc.). The provided DNA/RNA Shield inactivates infectious agents and is ideal for sample storage at ambient temperatures. Total RNA is eluted into 50 l of ZymoBIOMICS DNase/RNase-Free Water and is ready for any downstream application including Next-Gen Sequencing, RT-qPCR, etc.For automation scripts and support, eBinding Capacity:15 μg total RNA per 30 μl MagBinding Beads.Equipment Needed:Magnetic stand, heat block, liquid handler or robotic sample processor (user provided). Purity:High-quality RNA is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc. Recommended Materials:(sold separately) €“ 96-well Collection Plate (C2002; capacity is up to 1.2 ml/well), 96-Well Block (P1001; capacity is up to 2 ml/well), 96-well Elution Plate (C2003), Cover Foil (C2007), ZR-96 MagStand (P1005). Sample Preservation:DNA/RNA Shield lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures.Sample Types:Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA and RNA are efficiently isolated from 50mg of mammalian feces, 50mg soil, and 5-20mg (wet weight) of fungal bacterial cells, biofilms, water, and swabs. Size Limits:Total RNA including small/microRNAs 17nt. Storage:RNA eluted with DNase/RNase-Free Water (provided) can be stored frozen. The addition of RNase inhibitors is highly recommended for prolonged storage.Research or further manufacturing use only, not for food or drug use.

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