ZymoBIOMICS Spike-in Control I (High Microbial Load) consists of equal cell numbers of two bacteria strains, Imtechella halotolerans and Allobacillus halotolerans. When spiked into an unknown sample, this product will serve as an in situ positive control for DNA-sequencing-based (e.g. NGS-based) microbiome measurements. These two bacteria, Imtechella halotolerans (Gram-negative) and Allobacillus halotolerans (Gram-positive) represent different cell recalcitrance and can expose potential bias during DNA extraction. Moreover, with accurately quantified cell numbers, this standard enables absolute cell number quantification in microbiome measurements. Absolute Quantification: enables absolute cell number measurements using Next-Generation Sequencing in situ Quality Control: ensures each sample is quantified accurately Unique composition: comprised of two microbes alien to the human microbiomeStorage Temperature: -20CStorage Conditions: FreezerBiosafety: This product is not biohazardous as microbes have been fully inactivated. Impurity Level: < 0.01% foreign microbial DNA Reference Genomes: Complete reference genomes and 16S rRNA genesSample Storage: -80°C Storage Solution: DNA/RNA ShieldShipping Weight: 0.25 lbsShipping Dimensions: 4.00 x 2.00 x 2.00Research or further manufacturing use only, not for food or drug use.