ZymoPURE II Plasmid Maxiprep, 10 Preps

Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Maxiprep kit provides the fastest and simplest method available to efficiently isolate up to 1.2 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Plasmid Maxiprep kit can process up to 150 ml of culture in less than 20 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is bound onto a column with either a vacuum or a centrifuge instead of a lengthy gravity flow column. Additionally, there is no ethanol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 3 μg/μl), EndoZero, vaccine-grade, and transfection-ready. As an added convenience, the ZymoPURE II Plasmid Maxiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. Fastest: Simple 20 minute Maxipreps.Highest Yield: Purify up to 1.2mg of plasmid DNA directly from a spin-column.Ultra-Pure: EndoZero, vaccine grade, and transfection ready.
Storage Temperature: Room Temperature
Storage Conditions: Room Temperature
Applicable For: Transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, genome editing, and other sensitive applications. Binding Capacity: 1.2 mg Culture Input: 150 mlElution Volume: 200 μl Endotoxin Levels: 0.025 EU/μg Plasmid DNAEquipment: Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge.Format: Spin Column Processing Time: 20 min Purity: Typical Abs 260/280 1.8 and Abs 260/230 2.0 Recommended Sample Volume: 150 mlSize Range: Up to 200 kb Yield: 1.2 mg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized)Shipping Weight: 4.7501 lbs
Shipping Dimensions: 13.00 x 8.00 x 6.50
Research or further manufacturing use only, not for food or drug use.