ZymoPURE II Plasmid Midiprep, 25 Preps

Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Midiprep kit provides the fastest and simplest method available to efficiently isolate up to 400 μg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Midiprep kit can process up to 50 ml of culture in less than 20 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is bound onto a column with either a vacuum or a centrifuge instead of a lengthy gravity flow column. Additionally, there is no ethanol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 3 μg/μl), EndoZero, vaccine-grade, and transfection-ready. As an added convenience, the ZymoPURE II Plasmid Midiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.Fastest: Simple 20 minute Midipreps.Highest Yield: Purify up to 400 μg of plasmid DNA directly from a spin-column. Ultra-Pure: EndoZero, vaccine grade, and transfection ready.
Storage Temperature: Room Temperature
Storage Conditions: Room Temperature
Applicable For: Transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, genome editing, and other sensitive applications. Binding Capacity: 400 μg Culture Input: 50 ml Elution Volume: 100 μl Endotoxin Levels: 0.025 EU/μg Plasmid DNAEquipment: Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge Format: Spin ColumnProcessing Time: 20 minPurity: Typical Abs 260/280 1.8 and Abs 260/230 2.0Size Range: Up to 200 kbYield: 400 μg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized)Shipping Weight: 6.85 lbs
Shipping Dimensions: 13.00 x 8.00 x 15.00
Research or further manufacturing use only, not for food or drug use.