ZymoTaq qPCR Premix, 200 Rxns

ZymoTaq qPCR PreMix contains all the reagents needed to perform quantitative PCR and other molecular downstream analysis such as high-resolution melt (HRM) analysis of DNA methylation and other general real-time PCR assays. This premix features a hot-start DNA polymerase and buffer system optimized for the amplification of bisulfite-treated DNA. It also includes an intense dsDNA-specific fluorescent dye, SYTO 9, for sensitive real-time DNA quantification. This technology can also be found in the Femto Quantification Kits for low copy detection of DNA from human, bacteria, or fungi. Hot-start polymerase for easy setup at room temperature. Robust amplification of DNA for genotyping, SNP, and HRM analysis. Strong fluorescent signal for real-time and quantitative PCR assays.
Activity: 5' - 3' DNA polymerization Concentration: Reaction conditions at 1X (50 μl total volume) will contain 2 U of ZymoTaq DNA polymerase.Equipment: Real-time PCR can be performed on any instrument that has optical scanners that do not require passive reference dye. Storage: Store at -20°C for up to 12 months. Minimize exposure to light. Avoid repeated freeze/thawing of reagents. Unit Definition: One unit (U) enzyme of ZymoTaq DNA Polymerase is defined as the amount of enzyme required for the incorporation of 10 nmol dNTPs into an acid-insoluble form in 30 minutes at 72°C.Shipping Weight: 0.125 lbs
Shipping Dimensions: 6.00 x 5.00 x 2.00
Research or further manufacturing use only, not for food or drug use.