-Seq ATAC Library Kit, 12 preps

Zymo-Seq ATAC Library Kit provides a simple and streamlined workflow for Assay for Transposase Accessible Chromatin with high throughput Sequencing ATAC-Seq library preparation from mammalian cell and tissue input. This all-inclusive kit features an easy to follow protocol capable of preparing ATAC-Seq libraries from as little as 50,000 cells in as little as 4 hours with minimal hands on time.Initial cell lysis is gentle, ensuring that while the important nuclei are spun out of the lysed cell and collected, the contaminating mitochondrial DNA stays within the cell and is discarded. Next, the transposase, preloaded with Illumina adaptors, simultaneously fragments and tags tagments open chromatin regions in one rapid reaction. Finally, the tagmented DNA is indexed and amplified via PCR with UDI Tag Primer Set, generating high quality libraries that are ready to be sequenced on any Illumina instrument.Ready to Use: Preassembled buffers allow for lightning-fast library preparation in as little as 4 hours without compromising quality.Improved Performance: Prepare libraries with 7x less mitochondrial contamination, saving reads and increasing sequencing depth.Outstanding Consistency: Produce highly correlated replicates from both fresh and frozen samples.DNA Input: For optimal results, use 50,000 cells per technical replicate. Cells should present viability measure of the proportion of live cells within a population >80%. Recommend using cell counter to measure viability accurately.Equipment Required: Microcentrifuge, Biosafety cabinet, Cell counter, water bath, thermocycler, and thermoshaker with capacity to heat to 37C and shake at 1000 rpm We recommend USA Scientific Mixer HC with a thermo block that can fit 1.5 mL tubes and 2 mL Low Binding tubes.Library Storage: Libraries eluted in DNA Elution Buffer may be stored at 4C overnight or at -20C for long term storage.Processing Time: 4 hoursReagents Not Provided: Trypan blue, PBS, DNase I, cell media, trypsin, FBS, and DMSO.Sequencing Platform Compatibility: Libraries are compatible with Illumina sequencing platforms HiSeq, NextSeq, NovaSeq.Shipping Weight: 5.0001 lbsResearch or further manufacturing use only, not for food or drug use.