5-mC DNA ELISA Kit, 1 x 96

The 5-mC DNA ELISA Kit allows researchers to accurately quantitate 5-mC in any DNA sample in less than 3 hours. The kit features an Anti-5-Methylcytosine monoclonal antibody that is both sensitive and specific for 5-mC. The assay is compatible with a wide range of input DNA from vertebrate, plant, and microbial sources as well as methylated PCR amplicons and fragmented DNA. Percent 5-mC in a DNA sample can be accurately quantified from a standard curve generated with specially designed controls included with the kit. Also, the fast, streamlined workflow is ideal for high-throughput analyses. For high-throughput detection of global 5-methylcytosine (5-mC) in DNAThe streamlined workflow can be completed in less than 3 hours
Storage Temperature: -20C
Storage Conditions: Freezer
Detection: > 0.5% 5-mC per 100 ng DNAEquipment: Incubator and ELISA plate reader. A multi-channel pipettor is recommended. An automated plate washer may be used for blocking and wash steps.Input: This protocol is optimized for 100 ng input DNA/well. Compatible with DNA in the range of 10-200 ng/well. Sample Source: Purified genomic DNA, plasmid DNA, PCR amplification products, or DNA fragments in water, Tris-EDTA, or similar.Shipping Weight: 10.0626 lbs
Shipping Dimensions: 7.00 x 5.00 x 4.50
Research or further manufacturing use only, not for food or drug use.