His-Spin Protein Miniprep Kit, 50 Preps

The His-Spin Protein Miniprep provides a fast spin-column based purification technology for His-tagged proteins. Up to 1 mg of His-tagged protein can be purified in 5 minutes and eluted in as little as 100 μl of His-Elution Buffer. The purified protein is ultra-pure and can be used directly for enzymatic assays, protein biochemical analyses, SDS-PAGE and other sensitive applications. The straightforward spin, wash, and elute protocol dramatically simplifies protein purification and allows the end user to get results in minutes, not hours. Fast: Purify His-tagged proteins from cell lysates in less than 5 minutes. Simple: Prepare pure protein for small-scale studies using a spin-column. Convenient: No special instrumentation needed other than a benchtop microcentrifuge.
Storage Temperature: Room Temperature
Storage Conditions: Room Temperature
Affinity Matrix: Nickel charged agarose Binding Capacity: 1 mg Elution Method: Imidazole gradientElution Volume: 100 - 200 μl (150 μl is optimal)Principle of Technology: Based on a nickel-charged His-Affinity Gel (IMAC).Processing Time: 5 minutes Product Storage: Please store the His-Affinity Gel, His-Binding Buffer, His-Wash Buffer, and His-Elution Buffer at 4°C. The other components can be stored at room temperature.Protein Purity: Electrophoretically pure. Purified high-quality protein is suitable for enzyme kinetics, protein biochemical analyses, SDS-PAGE, and other applications.Required Equipment: Microcentrifuge Sample Type: Cell lysates or other complex protein mixtures containing His-tagged proteins.Shipping Weight: 1.25 lbs
Shipping Dimensions: 7.00 x 5.00 x 4.50
Research or further manufacturing use only, not for food or drug use.