-Seq Methyl Spike-in Control

Zymo-Seq Methyl Spike-in Control is comprised of six unique double-stranded synthetic amplicons 180-200 bp with distinct sequences derived from the E. coli K12 genome, and each amplicon represents a different CpG methylation level ranging from 0% to 100%. This control serves as an in situ control for next generation sequencing library preparations, providing an unbiased way of calculating the efficiency of the bisulfite conversion reaction. It can also be used to validate bioinformatics pipeline calibration by demonstrating a strong correlation between observed and expected methylation levels of the amplicons. Ensures reliable calculation of bisulfite conversion efficiency post library prep.Six unique amplicons with 0, 10, 25, 50, 75, and 100% methylation levels allow for a standard curve and robust data normalization.Compatible with various species except for E. coli and bisulfite sequencing library preparation methods. Concentration : 60 pg/l Amplicon # Methylation Level 1 0% 2 10% 3 25% 4 50% 5 75% 6 100% Source : Double-stranded synthetic amplicons derived from the E. coli genome.Storage : 20CShipping Weight: 0.05 lbsShipping Dimensions: 5.00 x 3.00 x 0.03Research or further manufacturing use only, not for food or drug use.